The Peptide Guide

Free Tool

Peptide reconstitution calculator

Three inputs — vial size, bacteriostatic water, target dose. Four outputs — concentration, injection volume, exact U-100 syringe units, and doses per vial. No account, no cost, no math errors.

Worked example

Vial size

10 mg

BAC water

2 mL

Desired dose

500 mcg

Result

Concentration 5 mg/mL · Draw 0.1 mL = 10 units on a U-100 syringe · 20 doses per vial

The math: 10 mg ÷ 2 mL = 5 mg/mL. 500 mcg ÷ 5,000 mcg/mL = 0.1 mL = 10 units. Want to understand it fully? Read the reconstitution guide.

Also in the app

Tirzepatide titration

The 2.5 → 15 mg trial schedule, mapped to your vial concentration.

Semaglutide dosing

STEP-schedule titration from 0.25 mg with per-step syringe units.

Retatrutide titration

Phase-2 escalation reference with weekly reminders.

Frequently asked questions

How does a peptide reconstitution calculator work?

It divides vial contents (mg) by water added (mL) to get concentration, then divides your target dose by that concentration to get the injection volume — converted to U-100 syringe units automatically.

Is the calculator really free?

Yes. The reconstitution calculator at My Peptide Protocol is free with no account required.

Does it handle GLP-1 titration schedules?

The app includes dedicated titration tools for semaglutide, tirzepatide, and retatrutide based on published trial schedules.

Why not build the calculator into this site?

The calculator lives in the companion app, where doses can also be logged and tracked against protocols — one tool, one data record.